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A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway.

Doherty EE, Adler BA, Yoon PH, Hsieh K, Loi K, Armbuster EG, Lahiri A, Bolling CS, Wilcox XE, Akkati A, Iavarone AT, Pogliano J, Doudna JA
bioRxiv [Preprint]. 2025 Apr 1:2025.03.28.646030. doi: 10.1101/2025.03.28.646030. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246058pBA1326Bacterial expression of RFP under control of the pJEX promoter
246059pBA1398Bacterial expression of T4 Acb1 under control of the pJex promoter
246060pBA1399Bacterial expression of T4 Acb2 under control of the pJex promoter
246061pBA1540Bacterial expression of 2TMbeta protein under the pJex promoter
246062pBA1543Bacterial expression of mutant T4 Acb2 (Y8A) under control of the pJex promoter
246063pBA1729Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori
246064pBA1739Bacterial expression of mCpol under control of the pTet promoter
246067pBA1802Bacterial expression of mutant T4 Acb1 (H44A,H113A) under control of the pJex promoter
246069pBA1866Bacterial expression of N4gp48 under control of the pJex promoter
246070ED_p_060ECOR31 mCpol bacterial expression under T7 promoter, C-term TEV-MBP-HIS10
246071ED_p_044aECOR31 mCpol bacterial expression under T7 promoter, N-term His10-MBP-TEV
246073ED_p_126Bacterial expression of ECOR31 2TMbeta residues 72-200 with N-terminal His-SUMO tag and co-expression of ECOR31 mCpol

Antibodies from Article