A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway.
Doherty EE, Adler BA, Yoon PH, Hsieh K, Loi K, Armbuster EG, Lahiri A, Bolling CS, Wilcox XE, Akkati A, Iavarone AT, Pogliano J, Doudna JA
bioRxiv [Preprint]. 2025 Apr 1:2025.03.28.646030. doi: 10.1101/2025.03.28.646030.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246059 | pBA1398 | Bacterial expression of T4 Acb1 under control of the pJex promoter |
| 246060 | pBA1399 | Bacterial expression of T4 Acb2 under control of the pJex promoter |
| 246062 | pBA1543 | Bacterial expression of mutant T4 Acb2 (Y8A) under control of the pJex promoter |
| 246064 | pBA1739 | Bacterial expression of mCpol under control of the pTet promoter |
| 246067 | pBA1802 | Bacterial expression of mutant T4 Acb1 (H44A,H113A) under control of the pJex promoter |
| 246069 | pBA1866 | Bacterial expression of N4gp48 under control of the pJex promoter |
| 246070 | ED_p_060 | ECOR31 mCpol bacterial expression under T7 promoter, C-term TEV-MBP-HIS10 |
| 246071 | ED_p_044a | ECOR31 mCpol bacterial expression under T7 promoter, N-term His10-MBP-TEV |