A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway.
Doherty EE, Adler BA, Yoon PH, Hsieh K, Loi K, Armbuster EG, Lahiri A, Bolling CS, Wilcox XE, Akkati A, Iavarone AT, Pogliano J, Doudna JA
bioRxiv [Preprint]. 2025 Apr 1:2025.03.28.646030. doi: 10.1101/2025.03.28.646030.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246058 | pBA1326 | Bacterial expression of RFP under control of the pJEX promoter |
| 246059 | pBA1398 | Bacterial expression of T4 Acb1 under control of the pJex promoter |
| 246060 | pBA1399 | Bacterial expression of T4 Acb2 under control of the pJex promoter |
| 246061 | pBA1540 | Bacterial expression of 2TMbeta protein under the pJex promoter |
| 246062 | pBA1543 | Bacterial expression of mutant T4 Acb2 (Y8A) under control of the pJex promoter |
| 246063 | pBA1729 | Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori |
| 246064 | pBA1739 | Bacterial expression of mCpol under control of the pTet promoter |
| 246067 | pBA1802 | Bacterial expression of mutant T4 Acb1 (H44A,H113A) under control of the pJex promoter |
| 246069 | pBA1866 | Bacterial expression of N4gp48 under control of the pJex promoter |
| 246070 | ED_p_060 | ECOR31 mCpol bacterial expression under T7 promoter, C-term TEV-MBP-HIS10 |
| 246071 | ED_p_044a | ECOR31 mCpol bacterial expression under T7 promoter, N-term His10-MBP-TEV |
| 246073 | ED_p_126 | Bacterial expression of ECOR31 2TMbeta residues 72-200 with N-terminal His-SUMO tag and co-expression of ECOR31 mCpol |