A versatile anti-CRISPR platform for opto- and chemogenetic control of CRISPR-Cas9 and Cas12 across a wide range of orthologs.
Brenker L, Aschenbrenner S, Bubeck F, Staykov K, Gebhardt C, Wolf B, Jendrusch M, Kroll AS, Becker J, Ambiel I, Fackler OT, Grimm D, Mathony J, Niopek D
Nucleic Acids Res. 2025 Jul 19;53(14):gkaf752. doi: 10.1093/nar/gkaf752.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246043 | CMV_AcrIIA5_AsLOV2-D41 | Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9 |
| 246044 | CMV_AcrIIA5_AsLOV2-N77 | Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9 |
| 246045 | CMV_AcrIIA5_cpGR2(GGS5)-D41 | Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9 |
| 246046 | CMV_AcrIIA5_cpGR2(GGS5)-N77 | Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9 |
| 246048 | AAV_CMV-AcrIIA5_cpGR2(GGS5)-N77 | Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9; contains ITRs for AAV packaging with Rep2 |
| 246049 | AcrVA1_AsLOV2-S103ΔELS | Expression of AcrVA1-AsLOV2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables optogenetic control of MbCas12a |
| 246050 | AcrVA1_cpGR2(GGS5)-S103ΔELS | Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12a |
| 246051 | AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2 | Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a |
| 246052 | CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66 | Expression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9 |
| 246053 | CMV_AcrIIC3_cpGR2(GGS5)-F59 | Expression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9 |
| 246054 | H1_sgRNA(GRIN2B)_SauCas9 | Expression of a sgRNA targeting the GRIN2B locus and SauCas9 under the same pol-III H1 promoter |
| 246055 | H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41) | Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before D41) linked through a P2A sequence, under the same pol-III H1 promoter |
| 246056 | H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(N77) | Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before N77) linked through a P2A sequence, under the same pol-III H1 promoter |