Programmable promoter editing for precise control of transgene expression.
Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, Love KS, Ploessl DS, Galloway KE
Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246326 | (203-bp-DIAL-YB_TATA)-mGL-BGH_pKG1525 | 203-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246327 | (loxPcon-DIAL-YB_TATA)-mGL-BGH_pKG2787 | loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa |
| 246328 | (27-bp-DIAL-YB_TATA)-mGL-BGH_pKG1524 | 27-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246329 | (79-bp-DIAL-YB_TATA)-mGL-BGH_pKG1523 | 79-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246332 | (nested-DIAL-YB_TATA)-mGL-BGH_pKG2876 | nested DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre and VCre recombinase |
| 246333 | (203-bp-DIAL-minCMV)-mGL-BGH_pKG3654 | 203-bp DIAL Reporter Plasmid with minCMV expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246334 | (203-bp-DIAL-SV40)-mGL-BGH_pKG3687 | 203-bp DIAL Reporter Plasmid with SV40 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246335 | (203-bp-DIAL-minCMV2)-mGL-BGH_pKG3680 | 203-bp DIAL Reporter Plasmid with minCMV2 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246336 | (203-bp-DIAL-JB42CAT5)-mGL-BGH_pKG3688 | 203-bp DIAL Reporter Plasmid with JB42CAT5 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246337 | (203-bp-DIAL-MLP)-mGL-BGH_pKG3681 | 203-bp DIAL Reporter Plasmid with MLP expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246338 | (203-bp-DIAL-CMV53)-mGL-BGH_pKG3682 | 203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246339 | (203-bp-DIAL-YB_TATA)-Halo-p53-BGH_pKG2745 | 203-bp DIAL Reporter Plasmid with YB_TATA expressing Halo-p53 in the presence of ZFa and editable by Cre recombinase |
| 246340 | (203-bp-DIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG2744 | 203-bp DIAL Reporter Plasmid with YB_TATA expressing mCherry-GS-HRasG12V in the presence of ZFa and editable by Cre recombinase |
| 246341 | (203-bp-DIAL-YB_TATA)-mGL-2A-mCherry-BGH_pKG2848 | 203-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern and mCherry in the presence of ZFa and editable by Cre recombinase |
| 246343 | LentiX_((203-bp-DIAL-YB_TATA)-mGL-BGH)rev_DIV_pKG2948 | 203-bp DIAL Reporter Lentivirus (with divergent iRFP670) expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase |
| 246344 | LentiX_((380-bp-DIAL)-mCh-HRasG12V-BGH)rev_DIV_pKG3848 | 380-bp DIAL (YB_TATA) Reporter Lentivirus (with divergent SNAP) expressing mCherry-HRasG12V in the presence of ZFa and editable by Cre recombinase |
| 246345 | LentiX_(loxPconDIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG3906 | loxPcon DIAL Reporter Lentivirus expressing mCherry-HRasG12V in the presence of ZFa |
| 246346 | LentiX_EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG2942 | ZFa Lentivirus expressing VP16-ZF43-2A-TagBFP from the EF1a promoter |
| 246347 | LentiX_EF1a-VP16-ZF37-2A-mCherry-BGH_pKG2943 | ZFa Lentivirus expressing VP16-ZF37-2A-mCherry from the EF1a promoter |
| 246348 | LentiX_EF1a-VP16-ZF37-2A-TagBFP-WPRE_pKG3675 | ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the EF1a promoter |
| 246349 | LentiX_PGK-VP16-ZF37-2A-TagBFP-WPRE_pKG3958 | ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the hPGK promoter |
| 246350 | pMXs-VP16-ZF37-2A-mCherry-WPRE_pKG2987 | ZFa Retrovirus expressing VP16-ZF37-2A-mCherry |
| 246351 | pMXs-VP16-ZF43-2A-TagBFP-WPRE_pKG2988 | ZFa Retrovirus expressing VP16-ZF43-2A-TagBFP |
| 246352 | pMXs-GIB-Cre-N-WPRE_pKG3943 | GIB-Cre Retrovirus expressing N-terminal giberellin inducible Cre |
| 246353 | pMXs-GIB-Cre-C-WPRE_pKG3944 | GIB-Cre Retrovirus expressing C-terminal giberellin inducible Cre |
| 246354 | pMXs-iCre-WPRE_pKG3028 | Retrovirus expression Cre recombinase |
| 246355 | EF1a-VP16-ZF37-2A-mCherry-BGH_pKG1501 | ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the EF1a promoter |
| 246356 | EF1a-VP16-ZF37-2A-TagBFP-BGH_pKG3223 | ZFa Plasmid expressing VP16-ZF37-2A-TagBFP from the EF1a promoter |
| 246357 | EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG1526 | ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the EF1a promoter |
| 246358 | (203-bp-TET-DIAL-YB-TATA)-mGL-bGH_pKG3015 | 203-bp TET-DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246359 | (loxPcon-TET-DIAL-YB-TATA)-mGL-bGH_pKG3016 | loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246360 | (90-bp-TET-DIAL-YB-TATA-VloxP)-mGL-bGH_pKG3017 | 90-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by VCre recombinase |
| 246361 | (380-bp-TET-DIAL-YB-TATA)-mGL-bGH_pKG3262 | 380-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246362 | (610-bp-TET-DIAL-YB-TATA)-mGL-bGH_pKG3263 | 610-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246363 | (203-bp-TET-DIAL-minCMV)-mGL-bGH_pDA028 | 203-bp DIAL Reporter Plasmid with minCMV expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246364 | (203-bp-TET-DIAL-minSV40)-mGL-bGH_pDA030 | 203-bp DIAL Reporter Plasmid with minSV40 expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246365 | (203-bp-TET-DIAL-CMV53)-mGL-bGH_pDA046 | 203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246366 | (203-bp-TET-DIAL-MLP)-mGL-bGH_pDA047 | 203-bp DIAL Reporter Plasmid with MLP expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase |
| 246369 | pIVT-PuroR-P2A-iCre-polyA_pKG03717 | IVT template for making modRNA encoding PuroR-P2A-iCre |
| 246371 | pIVT-mRuby2-P2A-PuroR_pKG02755 | IVT template for making modRNA encoding mRuby2-P2A-PuroR |
| 246372 | CAG-rtTA-2A-tagBFP-bGH_pKG3264 | Expression plasmid for rtTA-2A-tagBFP driven from the CAG promoter |
| 246373 | EFS-rtTA-2A-mRuby2-bGH_pKG2064 | Expression plasmid for rtTA-2A-mRuby2 driven from the EFS promoter |
| 246374 | EF1a-iRFP670-SV40_pKG726 | Expression plasmid for iRFP670 driven from the EF1a promoter. Co-transfection marker for transfection experiments. |
| 246375 | Multi-Janus-Spacer-1x_pKG1126 | Multi-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments. |
| 246376 | CMV-rtTA-BG-CMV-PuroR-BGH_pKG1701 | Expression plasmid for rtTA and PuroR |
| 246377 | TET-ZF37-mCherry-BGH_pKG01502 | ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the TET promoter |
| 246378 | TET-ZF43-TagBFP-BGH_pKG01531 | ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the TET promoter |
| 246379 | LentiX1-TET-VP16-ZF43-2A-tagBFP-bGH_DIVrtTA_pAMB282 | ZFa Lentivirus expressing VP16-ZF43-2A-TagBFP from the TET promoter with rtTA expressed divergently |