Adeno-Associated Viral Vector Resource for the RNA-Targeting Cas13d: A Comparison of High-Fidelity Variants, DjCas13d and hfCas13d
Back F, Sandoval A, Vu L, Hong VM, Bhaskara A, Rodriguez SR, O’Brien JT, Kolber BJ, Kroener S, Ploski JE
Mol Ther Methods Clin Dev, 20 Aug 2025, 101565
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192481 | pAAV-U6-DR30(SapI)-EFS-CasRx-T2A-mCherry-pA | To express CasRX and a gRNA |
| 192482 | pAAV-EFS-CasRx-pA | To express CasRx |
| 192483 | pAAV-0.4CaMKII-CasRx-T2A-mCherry-pA | To express CasRx |
| 192484 | pAAV-EF1a-CasRx-T2A-mCherry-pA | To express CasRx |
| 192485 | pAAV-U6-DR30(SapI)-CMV-intron-CasRx-pA | To express CasRX and a gRNA |
| 192487 | pAAV-CMV-intron-CasRx-pA | To express CasRx |
| 192488 | pAAV-U6-DR30(SapI)-EFS-CasRx-pA | To express CasRX and a gRNA |
| 192489 | pAAV U6-DR30(sapI)-0.5Syn-CasRX-mCherry-pA | To express CasRX and a gRNA |
| 192490 | pAAV U6-DR30(sapI)-0.4CaMKIIa-CasRX-mCherry-pA | To express CasRX and a gRNA |
| 192491 | pAAV-0.5Syn-CasRX-mCherry-pA | To express CasRx |
| 192492 | pAAV-U6-DR30(SapI)-0.5Syn-CasRx-pA | To express CasRX and a gRNA |
| 192493 | pAAV-U6-DR30(SapI)-CMV-intron-GFP-pA | To express CasRX and a gRNA |
| 192494 | pAAV-EFS-CasRx-T2A-mCherry-pA | To express CasRx |
| 192495 | pAAV-CMV-intron-DjCas13d-pA | To express DjCas13d |
| 192496 | pAAV-U6-DR30(SapI)-CMV-intron-MCS-pA | To express CasRX gRNA and contains MCS to insert coding region of interest |
| 192497 | pAAV-EFS-hfCas13d-T2A-mCherry-pA | To express hfCas13d |
| 192498 | pAAV-CMV-intron-DIO-DjCas13d-pA | To express DjCas13d in a Cre dependent manner |
| 192499 | pAAV-EFS-DjCas13d-T2A-mCherry-pA | To express DjCas13d |
| 192500 | pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-GFP-pA | To express DjCas13d compatible gRNA and GFP |
| 195864 | pAAV-CMV-intron-hfCas13d-pA | To express hfCas13d |
| 195865 | pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA | To express hfCas13d |
| 195866 | pAAV-CMV-intron-DIO-hfCas13d-pA | To express hfCas13d |
| 195867 | pAAV-2X(CMV-eBFP2-3XNLS-pA) | to express nuclear localized eBFP2 |
| 233025 | pAAV-EFS-CasRx-P2A-mCherry-pA | To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site |
| 233026 | pAAV-EFS-hfCas13d-P2A-mCherry-pA | To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site |
| 233027 | pAAV-EFS-DjCas13d-P2A-mCherry-pA | To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site |
| 233028 | pAAV-EFS-CasRx-HA-mCherry-pA | To Express HA tagged CasRX-mCherry fusion protein from the mammalian EFS promoter |
| 233029 | pAAV-EFS-hfCas13d-HA-mCherry-pA | To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter |
| 233030 | pAAV-EFS-DjCas13d-HA-mCherry-pA | To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter |
| 233031 | pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA | To express a DjCas13d-compatible gRNA |
| 233032 | pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa | To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome |
| 233033 | pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa | To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome |
| 233034 | pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa | To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome |
| 233035 | pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa | To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter |
| 233036 | pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa | To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter |
| 233037 | pAAV-EF1a-hfCas13d-pa | To express hfCas13d from a EF1a promoter |
| 233038 | pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter |
| 233039 | pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter |
| 233045 | pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site. |
| 233046 | pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA | To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site. |