Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for Anhydride-Mediated Cell Ligation.
Lim SYT, Keeble AH, Howarth MR
ACS Chem Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246647 | AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003 | Expresses AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003 in bacterial cells for calcium-induced NeissLock protein ligation |
| 246648 | His6-SUMO-SPMc-SpyCatcher003-Ctag | Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligation |
| 246649 | tPA-TGFa-SPMn-SpyTag003 | Expresses tPA-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR) |
| 246650 | Neo-2/15-TGFa-SPMn-SpyTag003 | Expresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR) |