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DNAJC13 localization to endosomes is opposed by its J domain and its disordered C-terminus.

Adoff H, Novy B, Holland E, Lobingier BT
Mol Biol Cell. 2025 Sep 1;36(9):ar114. doi: 10.1091/mbc.E24-12-0575. Epub 2025 Jul 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246660pEGFP-C1-DNAJC13(FL)Mammalian expression of full length, eGFP tagged DNAJC13
246661pEGFP-C1-DNAJC13(2198t)Mammalian expression of truncated, eGFP tagged DNAJC13
246662pEGFP-C1-DNAJC13(ylt1)Mammalian expression of mutant, eGFP tagged DNAJC13
246663pEGFP-C1-DNAJC13(hpd)Mammalian expression of DnaJ domain mutant, eGFP tagged DNAJC13
246664pEGFP-C1-DNAJC13(2198t+hpd)Mammalian expression of dual mutant, eGFP tagged DNAJC13
246665pEGFP-C1-DNAJC13(ylt1+hpd)Mammalian expression of dual mutant, eGFP tagged DNAJC13
246666pEGFP-C1-DNAJC13(351t)Mammalian expression of truncated, eGFP tagged DNAJC13 (PH-like domain only)
246667pEGFP-C1-DNAJC13(351t)-dimerMammalian expression of truncated, eGFP tagged DNAJC13 (PH-like domain only), with exogenous dimerization system

Antibodies from Article