CHIP protects lysosomes from CLN4 mutant-induced membrane damage.
Lee J, Chin N, Zou J, Mazli WNAB, Jarnik M, Saidi L, Xu Y, Jeong E, Suh J, Replogle J, Ward ME, Bonifacino JS, Zheng W, Hao L, Ye Y
Nat Cell Biol. 2025 Aug 25. doi: 10.1038/s41556-025-01738-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246738 | pCMV6 CHIP delU-box-Myc | Mammalian expression of human CHIP with U-box domain deleted, and a myc-tag on its C-terminus |
| 246739 | pCMV6 CHIP-Myc | Mammalian expression of human CHIP wildtype, and a myc-tag on its C-terminus |
| 246740 | pCMV6 FLAG-DNAJC5 | Mammalian expression of human DNAJC5 with L116 deleted, and a FLAG tag on its N-terminus |
| 246741 | pCMV6 FLAG-DNAJC5 delL116 | Mammalian expression of human DNAJC5 with L115R mutation, and a FLAG tag on its N-terminus |
| 246742 | pCMV6 FLAG-DNAJC5 L115R | Mammalian expression of human DNAJC5 with L115R mutation, and a FLAG tag on its N-terminus |
| 246743 | pGEX GST-tev-hDNAJC5 | Bacterial expression of human DNAJC5 wildtype with GST and a cleavage site for TEV protease on its N-terminus |
| 246744 | pGEX GST-tev-hDNAJC5 delL116 | Bacterial expression of human DNAJC5 L116 deleted with GST and a cleavage site for TEV protease on its N-terminus |
| 246745 | pGEX GST-tev-hDNAJC5 L115R | Bacterial expression of human DNAJC5 L115R with GST and a cleavage site for TEV protease on its N-terminus |
| 246746 | pLenti mKeima | Mammalian and lentiviral expression of insert with a mKeima tag on its N-terminus |
| 246751 | sh-hCHIP | Mammalian and lentiviral expression of shRNA targeting human CHIP (Stub1) |