Self-splicing RNA circularization facilitated by intact group I and II introns.
Shen Y, Li B, Dong L, Tang W, Ren J, Chen F, Zheng W, Yu Y, Gao L, Wei W
Nat Commun. 2025 Aug 10;16(1):7376. doi: 10.1038/s41467-025-62607-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246753 | CIRC-Scarless-Ana-CVB3 IRES split 403-mCherry | Generate scarless circRNA with CVB3 IRES |
| 246754 | CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI | RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI. |
| 246755 | CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI | RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI. |
| 246756 | CIRC-V1-Tet-CVB3 IRES-EGFP-BbsI | RNA circularization with CIRC using Tet intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI. |
| 246757 | CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI | RNA circularization with CIRC using Azo intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI. |