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Direct measurements of luminal Ca2+ with endo-lysosomal GFP-aequorin reveal functional IP3 receptors.

Calvo B, Torres-Vidal P, Delrio-Lorenzo A, Rodriguez C, Aulestia FJ, Rojo-Ruiz J, Callejo B, McVeigh BM, Keller M, Grimm C, Oorschot V, Moiseenkova-Bell V, Yule DI, Garcia-Sancho J, Patel S, Alonso MT
J Cell Biol. 2025 Dec 1;224(12):e202410094. doi: 10.1083/jcb.202410094. Epub 2025 Nov 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246796pHSV-ELGABioluminescent endolysosomal Ca2+ indicator
246926pHSV-ELGA1Bioluminescent endolysosomal Ca2+ indicator lower Ca2+ affinity

Antibodies from Article