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Boolean Logic-Based Controlled Release of Bioactive Proteins with Diversified Inputs.

Ross ML, Gharios R, Kottantharayil S, Li A, DeForest CA
Angew Chem Int Ed Engl. 2025 Nov 10:e19925. doi: 10.1002/anie.202519925. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246944EGF-A-SpyTagExpresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
246945bla-A OR T-SpyTagExpresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
246946NanoLuc-A AND T-SpyTagExpresses multifunctional construct for AND-gated release of NanoLuciferase (NanoLuc) from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.
246947TrxA-(A AND C) OR T-SpyTagExpresses multifunctional construct for (AND)OR-gated release of ThioredoxinA from materials in response to TEV OR (sortase eSrtA(2A9) AND HRV-3C) treatment. Contains a SpyTag for material tethering.
246948Neo-A OR T OR C-SpyTagExpresses multifunctional construct for OR OR-gated release of Neoleukin (Neo) from materials in response to sortase eSrtA(2A9) OR TEV OR HRV-3C treatment. Contains a SpyTag for material tethering.
246949mGL-A AND T AND C-SpyTagExpresses multifunctional construct for AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV AND HRV-3C treatment. Contains a SpyTag for material tethering.
246950mCherry-PhoCl AND A-SpyTagExpresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.

Antibodies from Article