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Phage susceptibility to a minimal, modular synthetic CRISPR-Cas system in Pseudomonas aeruginosa is nutrient dependent.

Elliott JFK, Cozens K, Cai Y, Waugh G, Watson BN, Westra E, Taylor TB
Philos Trans R Soc Lond B Biol Sci. 2025 Sep 4;380(1934):20240473. doi: 10.1098/rstb.2024.0473. Epub 2025 Sep 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
246943pUC18T-mini-Tn7T-Gm-CCPlasmid strain containing pUC18T-mini-Tn7T-Gm-CC01 plasmid, which will insert a nonsense spacer version of the synthetic CRISPR-Cas system from P. aeruginosa PA14 into the genome at the att-Tn7 site.

Antibodies from Article