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PERRC: Protease Engineering with Reactant Residence Time Control.

Nelson S, Gaza J, Ajayebi S, Masse R, Pho R, Scutero C, Martinusen S, Long L, Menezes A, Perez A, Denard C
ACS Synth Biol. 2025 Jun 20;14(6):2241-2253. doi: 10.1021/acssynbio.5c00154. Epub 2025 May 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247042TEVESNp_pRK793pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVESNp insert.
247672TEVEp_pRK793pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVEp insert based on Yi et al., 2013.

Antibodies from Article