Repurposing base editors for targeted knockin and simultaneous multiplex knockouts to generate allo-CAR T cells with minimal translocations.
Glaser V, Becker LJ, Fuster-Garcia C, Aird EJ, Huth L, Nitulescu AM, Pu Y, Kassing I, Hartmann LM, Flugel CL, Karklins R, Shaji S, Pouzolles M, Stein M, Andrieux G, Corn JE, Cathomen T, Volk HD, Reinke P, Kath J, Wagner DL
Mol Ther. 2026 Jul 20:S1525-0016(26)00602-7. doi: 10.1016/j.ymthe.2026.07.034.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247056 | BEKI_pUC19_TRAC_CD19-CAR | Encodes HDR-template for base editor mediated knock-in (BEKI) of a CD19-CAR into human TRAC locus. With this strategy, common (nCas9-derived) base editors can be repurposed for targeted knock-in. |
| 247057 | BEKI_pTWIST_CD3z_CD19-truncCAR | Encodes HDR-template for base editor mediated knock-in (BEKI) of a truncated CD19-CAR into human CD3z (CD247) gene. In-frame gene fusion results in expression of full-length CAR. |