Continuous evolution of a halogenase enzyme with improved solubility and activity for sustainable bioproduction.
Pulschen AA, Booth J, Satanowski A, Soudy C, Caro-Astorga J, Ather O, Patel N, Alidoust A, Aoudjane S, Nematollahi L, DeBenedictis E
Nat Commun. 2026 Mar 24. doi: 10.1038/s41467-026-70981-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247093 | pJB21x05 | Expression of RgnTDC |
| 247094 | pJB15x02 | In vivo expression of wild-type Enterocin RJ-11 |
| 247095 | pJB15x06v2 | In vivo expression of Enterocin RJ-11 (W30 TAG) |
| 247096 | pJB18x02v1 | Production of SUMO-Enterocin RJ-11 W307-Cl |
| 247097 | pAP65 | Contains WT RebH |
| 247098 | pAP113 | Contains RebHEvo4 |
| 247099 | pAP54 | Contains Fre-L3-RebH (WT) |
| 247100 | pAP114 | Contains Fre-L3-RebHEvo4 |
| 247101 | pAP79 | Contains ChPheRS4*S333C |
| 247102 | pAP80 | Contains ChPheRS4*S333C, M490I |
| 247103 | pAP70 | Contains pIII*1TAG + Fre |