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The IQ-compete assay for measuring mitochondrial protein import efficiencies in living yeast cells.

Hoffman Y, Egeler A, Rodl S, Herrmann JM
FEBS Lett. 2025 Oct 25. doi: 10.1002/1873-3468.70206. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247176IQ-compete reporter (integrating)Plasmid for genomic integration of the IQ-compete reporter (GFP-TCS-Quencher-CL1) into the YPRCΔ15 locus of yeast cells.
247217IQ-compete reporter (episomal)Single-copy plasmid expressing the IQ-compete reporter (GFP-TCS-Quencher-CL1).
247218DHFR-TEV (integrating)Plasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.
247219Atp5-DHFR-TEV (integrating)Plasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.
247220Oxa1-DHFR-TEV (integrating)Plasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Antibodies from Article