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All-optical voltage interrogation for probing synaptic plasticity in vivo.

Carolan J, Land MA, Lu X, Beau M, Kostadinov D, St-Pierre F, Clark BA, Hausser M
Nat Commun. 2025 Oct 3;16(1):8834. doi: 10.1038/s41467-025-63867-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247180pAAV-CaMKIIa-JEDI-2Psub-WPREGenetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa
247186pcDNA3.1/Puro-CAG-JEDI-2PsubGenetically encoded voltage indicator (GEVI) JEDI-2Psub expressed under strong mammalian promoter (CAG)
247223pAAV-CaMKIIa-JEDI-2Psub-Kv-WPRESoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa
247224pAAV-EF1a-DIO-JEDI-2Psub-WPREDouble floxed genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector under the control of the mammalian promoter (EF1a)
247323pAAV-EF1a-DIO-JEDI-2Psub-Kv-WPREDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector expressed under the mammalian promoter (EF1a)

Antibodies from Article