Megabase-scale human genome rearrangement with programmable bridge recombinases.

Perry NT, Bartie LJ, Katrekar D, Gonzalez GA, Durrant MG, Pai JJ, Fanton A, Martins JQ, Hiraizumi M, Ricci-Tam C, Nishimasu H, Konermann S, Hsu PD
Science. 2025 Sep 25:eadz0276. doi: 10.1126/science.adz0276. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
247248	pNP2314 [Cloning backbone, Gluc/Cluc Inversion Activity Reporter]	Cloning backbone for measuring bridge recombinase mediated inversion
247249	pNP2321 (Cloning backbone, bRNA + recombinase]	Cloning backbone for cloning bridge recombinase and bridge RNA
247250	pNP2322 [Cloning backbone, mCherry Inversion Activity Reporter]	Cloning backbone for measuring bridge recombinase mediated inversion
247251	pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]	Measure ISCro4 activity via luciferase expression
247252	pNP2390 [pNP2322 - ISCro4 Donor + ISCro4 Target-RTG-Matched]	Measure ISCro4 activity via mCherry expression
247253	pNP2885 [pNP2922 - ISCro4 TBL1, WT]	Express ISCro4 recombinase and bridge RNA
247254	pNP2916 [Cloning backbone, bRNA Landing Pad]	Cloning backbone for cloning bridge RNA
247256	pNP2987 [pNP2922 - ISCro4 TBL3 (WT) + ISCro4 Recombinase]	Express ISCro4 recombinase and bridge RNA
247257	pNP3254 [pNP2922 - ISCro4 Enhanced DBL (DBL3, WT) + ISCro4 Recombianse (WT]	Express ISCro4 recombinase and bridge RNA
247258	pNP3386 [For use in genome insertion and off-target assessment]	Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI
247259	pNP3396 [pNP2922 - ISCro4 DBL1 (WT) + ISCro4 Recombinase (WT)]	Express ISCro4 recombinase and bridge RNA
247260	pNP3413 [pNP2922 - ISCro4 TBL2 (WT) + ISCro4 Recombinase (WT)]	Express ISCro4 recombinase and bridge RNA
247261	pNP3414 [pNP2922 - ISCro4 Enhanced TBL (TBL4, WT) + ISCro4 Recombinase (WT)]	Express ISCro4 recombinase and bridge RNA
247262	pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]	Express ISCro4 recombinase and bridge RNA
247263	pNP3440 [pNP2922 - ISCro4 bRNA (TBL2, WT + DBL1, WT) + ISCro4 Recombinase (WT)]	Express ISCro4 recombinase and bridge RNA
247264	pNP3613 [Cloning backbone, recombinase]	Cloning backbone for cloning bridge recombinase
247266	pNP3614-MM168 [pNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)]	Express ISCro4 recombinase
247267	pNP3620 [pNP2916 - ISCro4 bRNA (TBL2, WT + DBL1, WT)]	Express ISCro4 bridge RNA