Secretome translation shaped by lysosomes and lunapark-marked ER junctions.
Choi H, Liao YC, Yoon YJ, Grimm J, Wang N, Lavis LD, Singer RH, Lippincott-Schwartz J
Nature. 2025 Nov 5. doi: 10.1038/s41586-025-09718-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247296 | pCMV-LAMP1-iLID-Halo | Mammalian expression of LAMP1-iLID-Halo fusion for light-induced recruitment to late endosomes/lysosomes. |
| 247297 | pCMV-mCherry-sspB-Sec61 | Mammalian expression of mCherry-sspB-Sec61 fusion; for optogenetic control of protein recruitment to the ER |
| 247298 | pPB-cytERM-SUNTAG-MS2 | Mammalian expression of an ER-specific SUNTAG reporter; piggyBac vector to express cytERM-SUNTAG MS2 |
| 247299 | pPB-CrPV-IRES-cytERM-SUNTAG-MS2 | Mammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation. |