Yong Tian Lab: Engineering a CRISPR-associated IscB system for developing miniature genome-editing tools in human cells and mouse embryos
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247329 | enDelIscB | Vector encoding human codon-optimized engineered DelIscB driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter |
| 247330 | enDelIscB-T5E | Vector encoding human codon-optimized engineered DelIscB fused with T5E at C-terminal driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter |
| 247331 | ICBE | Vector encoding human codon-optimized engineered DelIscB nickase fusion with APOBEC3A and UGI driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter |
| 247332 | IABE | Vector encoding human codon-optimized engineered DelIscB nickase fusion with TadA8e driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter |