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A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications

Lee P-W, Mottaghi SS, Lugnier MG, Maerkl SJ
bioRxiv 2025.06.18.660355 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247367pQE30-His-LZ3B-T7RNAPExpress LZ3B-T7RNAP that dimerize with LZ3A
247369pQE30-His-spAb-T7 RNAPExpress spAb-T7 RNAP that bind to FC region of human IgG
247372pET-21(+)-His-zf438-NB23Express zf438-NB23 that bind to SARS-CoV-19 Spike protein cooperatively with NB15-T7 RNAP.
247374pQE30-His-zfAAA-T7RNAPThe plasmid encode N-terminal his tagged zf438-T7 RNAP that pair with pzfAAA-d1T7 promoter

Antibodies from Article