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MRTF-dependent cytoskeletal dynamics drive efficient cell cycle progression.

Nielsen JC, Benito-Jardon M, Petrela NC, Diring J, Bellamy S, Treisman R
J Cell Sci. 2025 Nov 7:jcs.264444. doi: 10.1242/jcs.264444. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247436pCW-MRTFA-HAsiRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
247437pCW-Rho(G14V)-HAHA-tagged human RhoA(G14V) cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
247438pCW-CDK1-HAHA-tagged mouse CDK1 cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
247439pCW-CKS2-HAHA-tagged mouse CKS2 cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
247440pCW-Myoferlin-HAHA-tagged human Myoferlin cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Antibodies from Article