An optimised system for rapid auxin-inducible protein degradation in budding yeast
Hubbe P, Sharma C, Pajonk O, Peters N, Guschtschin-Schmidt N, Friemel N, Schuck S
bioRxiv 2025.09.24.678194
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247600 | pRS405-Padh-AtAFB2 | Expression of AtAFB2 |
| 247601 | pFA6a-mAID-mNeon-HIS3MX6 | Yeast gene tagging with mAID |
| 247602 | pFA6a-mAID-mNeon-kITRP1 | Yeast gene tagging with mAID |
| 247603 | pRS405-Padh-OsTIR1(F74G) | Expression of OsTIR1 |
| 247604 | pRS406-Padh-OsTIR1(F74G) | Expression of OsTIR1 |
| 247605 | pNH605-Padh-OsTIR1(F74G) | Expression of OsTIR1 |
| 247606 | pNH604-Padh-OsTIR1(F74G) | Expression of OsTIR1 |
| 247607 | pFA6a-mIAA7-mNeon-HISMX6 | Yeast gene tagging with mIAA7 |
| 247608 | pFA6a-mIAA7-mNeon-kITRP1 | Yeast gene tagging with mIAA7 |
| 247609 | pNH605-Padh-OsTIR1 | Expression of OsTIR1 |
| 247610 | pNH605-Padh-OsTIR1(F74A) | Expression of OsTIR1 |
| 247611 | pNH605-Padh-AtAFB2 | Expression of AtAFB2 |
| 247612 | pNH605-Padh-AtAFB2(F74G) | Expression of AtAFB2 |
| 247613 | pNH605-Padh-AtAFB2(F74A) | Expression of AtAFB2 |
| 247614 | pFA6a-mIAA7-3xFLAG-HIS3MX6 | Yeast gene tagging with mIAA7 |
| 247615 | pFA6a-mIAA7-3xFLAG-klTRP1 | Yeast gene tagging with mIAA7 |
| 247616 | pFA6a-AID*-mNeon-HIS3MX6 | Yeast gene tagging with AID* |
| 247617 | pRS303K-Pgpd-OsTIR1(F74G) | Expression of OsTIR1 |
| 247618 | pRS303K-Padh-OsTIR1(F74G) | Expression of OsTIR1 |
| 247619 | pRS303K-Prnr1-OsTIR1(F74G) | Expression of OsTIR1 |
| 247620 | pFA6a-mIAA7-3xFLAG-kanNP1 | Yeast gene tagging with mIAA7 |