Characterization of Fluorescent Reporters for Flow Cytometry-Based Single-Cell Studies in Saccharomyces cerevisiae.
Evans S, Tominaga M, Lu Z, Bandari NC, McDonnell L, Wang C, Speight RE, Ishii J, Vickers CE, Peng B
ACS Synth Biol. 2025 Sep 19;14(9):3804-3812. doi: 10.1021/acssynbio.5c00432. Epub 2025 Sep 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223835 | pILFPZ1A9 | Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae |
| 223836 | pILFPQ13BE5 | Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae |
| 223837 | pILFPQ13A7 | Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae |
| 223838 | pILFPQ13B7 | Expressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiae |
| 223839 | pILFPmTagBFP2FrFAST | Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae |