WAChRs are excitatory opsins sensitive to indoor lighting
Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A
bioRxiv 2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247723 | pAAV-EF1a>WAChR_1f-G-mScarlet3-Soma-E; WPRE3-BGH polyA; AmpR | Constitutive expression of WAChR-1f opsin fusion protein: {WAChR-1f}-{Kir2.1 Golgi tag}-{mScarlet3}-{Kv2.1 soma tag}-{Kir2.1 ER tag} |
| 247724 | pAAV-EF1a>WAChR_1m-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR | Constitutive expression of WAChR-1m opsin fusion protein: {WAChR-1m}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag} |
| 247725 | pAAV-EF1a>WAChR_1s-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR | Constitutive expression of WAChR-1s opsin fusion protein: {WAChR-1s}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag} |
| 247727 | pAAV-Syn1>WAChR_1m-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR | Neuron-specific expression of WAChR-1m opsin fusion protein: {WAChR-1m}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag} |
| 247728 | pAAV-EF1a>WAChR_1m-G-Soma-E-HA-P2A-mScarlet; WPRE3-BGH polyA; KanR | Constitutive bicistronic expression via P2A cleavage: {WAChR-1m}-{Kir2.1 Golgi tag}-{Kv2.1 soma tag}-{Kir2.1 ER tag}-{HA tag} + {mScarlet} |