Cell-selective multiplexed bioorthogonal noncanonical amino acid tagging for nascent proteomics.
Loynd C, Singha Roy SJ, Canarelli SE, Bak DW, Sundaresh B, Babbitz Z, van Opijnen T, Weerapana E, Chatterjee A
Nat Chem Biol. 2025 Oct 2. doi: 10.1038/s41589-025-02039-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231129 | pBK-EcTrpRS(H14) | Expresses mutant E. coli TrpRS from a weak glnS promoter for proteome-wide incorporation of tryptophan analogues. |
| 231130 | pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G) | Expresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues. |
| 231131 | pBBR1-EcTrpRS(H14) | Expresses mutant E. coli TrpRS from a derepressed lac promoter for proteome-wide incorporation of tryptophan analogues in E. coli/K. pneumoniae. |
| 231132 | pBBR1-EcTyrRS(VSMA,D165G) | Expresses mutant E. coli TyrRS from a derepressed lac promoter for proteome-wide incorporation of tyrosine in E. coli/K. pneumoniae. |
| 231133 | pSGAb/pEvol-AbTrpRS(H14) | Expresses mutant A. baumannii TrpRS from an inducible lac promoter for proteome-wide incorporation of tryptophan analogues. |