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An engineered cysteine sensor optimized for high-throughput screening identifies regulators of intracellular thiol levels.

Abrams RPM, Donahue RG, Ma J, Mao Y, Diolaiti ME, Ashworth A
Cell Chem Biol. 2025 Nov 10:S2451-9456(25)00343-5. doi: 10.1016/j.chembiol.2025.10.006. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247756pLVX_cpCys203Lentiviral expression of the cpCys203 FRET-based cysteine sensor in mammalian cells
247757pLVX_ECFPLentiviral expression of ECFP in mammalian cells
247758pLVX_VenusLentiviral expression of Venus in mammalian cells
247759pcDNA3.1_cpCys203mammalian expression of the cpCys203 FRET-based cysteine sensor
247760pcDNA3.1_ECFPmammalian expression of ECFP
247761pcDNA3.1_Venusmammalian expression of Venus
247771pET29b_cpCys203bacterial expression of cpCys203 FRET-based cysteine sensor

Antibodies from Article