UBR5 forms ligand-dependent complexes on chromatin to regulate nuclear hormone receptor stability.
Tsai JM, Aguirre JD, Li YD, Brown J, Focht V, Kater L, Kempf G, Sandoval B, Schmitt S, Rutter JC, Galli P, Sandate CR, Cutler JA, Zou C, Donovan KA, Lumpkin RJ, Cavadini S, Park PMC, Sievers Q, Hatton C, Ener E, Regalado BD, Sperling MT, Slabicki M, Kim J, Zon R, Zhang Z, Miller PG, Belizaire R, Sperling AS, Fischer ES, Irizarry R, Armstrong SA, Thoma NH, Ebert BL
Mol Cell. 2023 Aug 3;83(15):2753-2767.e10. doi: 10.1016/j.molcel.2023.06.028. Epub 2023 Jul 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247926 | pZucchini2_SFFV-RARA-eGFP-IRES-mCherry_PuroR | Overexpression vector for eGFP tagged RARA or NR1B1. Monitor post-translational degradation of RARA via EGFP:mCherry ratio. Contains puromycin-resistance selection marker. |
| 247928 | pSquash2_SFFV-eGFP-NR3C1-IRES-mCherry_PuroR | Overexpression vector for eGFP tagged GR or NR3C1. Monitor post-translational degradation of GR via EGFP:mCherry ratio. Contains puromycin-resistance selection marker. |
| 247930 | pZucchini2_SFFV-ESR1-eGFP-IRES-mCherry_PuroR | Overexpression vector for eGFP tagged ESR1 or ERα. Monitor post-translational degradation of ERα via EGFP:mCherry ratio. Contains puromycin-resistance selection marker. |