Complete computational design of high-efficiency Kemp elimination enzymes.
Listov D, Vos E, Hoffka G, Hoch SY, Berg A, Hamer-Rogotner S, Dym O, Kamerlin SCL, Fleishman SJ
Nature. 2025 Jul;643(8074):1421-1427. doi: 10.1038/s41586-025-09136-2. Epub 2025 Jun 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247996 | pET28-hisSumo-KE-Des27 | Expression of synthetic Kemp eliminase (Des27) |
| 247997 | pET28-hisSumo-KE-Des27.1 | Expression of synthetic Kemp eliminase (Des27.1) |
| 247998 | pET28-hisSumo-KE-Des27.2 | Expression of synthetic Kemp eliminase (Des27.2) |
| 247999 | pET28-hisSumo-KE-Des27.3 | Expression of synthetic Kemp eliminase (Des27.3) |
| 248000 | pET28-hisSumo-KE-Des27.4 | Expression of synthetic Kemp eliminase (Des27.4) |
| 248001 | pET28-hisSumo-KE-Des27.5 | Expression of synthetic Kemp eliminase (Des27.5) |
| 248002 | pET28-hisSumo-KE-Des27.6 | Expression of synthetic Kemp eliminase (Des27.6) |
| 248003 | pET28-hisSumo-KE-Des27.7 | Expression of synthetic Kemp eliminase (Des27.7) |
| 248004 | pET28-hisSumo-KE-Des27.9 | Expression of synthetic Kemp eliminase (Des27.9) |
| 248005 | pET28-hisSumo-KE-Des27.10 | Expression of synthetic Kemp eliminase (Des27.10) |
| 248006 | pET28-hisSumo-KE-Des27.11 | Expression of synthetic Kemp eliminase (Des27.11) |
| 248007 | pET28-hisSumo-KE-Des27.12 | Expression of synthetic Kemp eliminase (Des27.12) |
| 248008 | pET28-hisSumo-KE-Des27.13 | Expression of synthetic Kemp eliminase (Des27.13) |
| 248009 | pET28-hisSumo-KE-Des61 | Expression of synthetic Kemp eliminase (Des61) |
| 248010 | pET28-hisSumo-KE-Des61.1 | Expression of synthetic Kemp eliminase (Des61.1) |
| 248011 | pET28-hisSumo-KE-Des27.7_ModularAssembly | Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly) |
| 248012 | pET28-hisSumo-KE-Des27.7_PROSS | Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS) |
| 248013 | pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite | Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design) |
| 248014 | pET28-hisSumo-KE-Des27.7_noCoreStab | Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability) |
| 248016 | pET28-hisSumo-KE-Des27.7_F113L | Expression of synthetic Kemp eliminase (Des27.7 F113L) |