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Inhibition of RAS-driven signaling and tumorigenesis with a pan-RAS monobody targeting the Switch I/II pocket.

Wallon L, Khan I, Teng KW, Koide A, Zuberi M, Li J, Ketavarapu G, Traaseth NJ, O'Bryan JP, Koide S
Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2204481119. doi: 10.1073/pnas.2204481119. Epub 2022 Oct 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248049pHBT-HRAS174To express His6 tag-Avi tag-TEV cleavage site-HRAS (residues 1-174) in E. coli, under the T7 promoter.
248050pHBT-KRAS174To express His6 tag-Avi tag-TEV cleavage site-KRAS (residues 1-174) in E. coli, under the T7 promoter.
248051pHBT-NRAS174To express His6 tag-Avi tag-TEV cleavage site-NRAS (residues 1-174) in E. coli, under the T7 promoter.

Antibodies from Article