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Genetically encoded tool for manipulation of ATP/ADP ratio in human cells.

Ekvik AE, Kober MM, Titov DV
bioRxiv [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 10.1101/2025.08.12.670003. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248065pLVX-TetOne-bsd-atpALentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.
248066pLVX-TetOne-bleo-atpDLentiviral expression of beta subunit of ATPGobble. Includes ampR and bleoR (zeocin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for active ATPGobble.
248067pLVX-TetOne-puro-atpGLentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.
248068pLVX-TetOne-bleo-atpD-K155QLentiviral expression of a catalytically inactive mutant of the beta subunit of ATPGobble. Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for a LOF control.

Antibodies from Article