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Synaptobrevin-2 disease variants reveal spatial constraints within the presynaptic active zone.

Guzikowski NJ, Bagatelas ED, Shin OH, Khan YA, Esquivies L, Alten B, Brunger AT, Kavalali ET
Proc Natl Acad Sci U S A. 2025 Nov 4;122(44):e2507347122. doi: 10.1073/pnas.2507347122. Epub 2025 Oct 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248132pFUW-Syb2-A67PMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with A67P mutation
248133pFUW-Syb2-E78AMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with E78A mutation
248134pFUW-Syb2-F77SMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with F77S mutation
248135pFUW-Syb2-G73WMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with G73W mutation
248136pFUW-Syb2-R56LMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with R56L mutation
248137pFUW-Syb2-R56XMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with R56X mutation
248138pFUW-Syb2-S75PMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with S75P mutation
248139pFUW-Syb2-V43delMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with V43del deletion
248140pFUW-Syb2-I45delMammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with I45del deletion

Antibodies from Article