FCRL3 is an immunoregulatory receptor that restrains the activation of human memory T lymphocytes

Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G, Monticelli S
J Exp Med (2026) 223 (1): e20242474. Article

Plasmids from Article

ID	Plasmid	Purpose
248148	pLVX-EF1_-FCRL3-IRES-puro	Lentiviral vector encoding human FCRL3
248149	pLVX-EF1_-EGFR-FCRL3-93aa-Chimera-puro	Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-687; first 93 residues), fused to the extracellular domain of EGFR.
248150	pLVX-EF1_-EGFR- FCRL3-50aa-Chimera-puro	Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-644; first 50 residues), fused to the extracellular domain of EGFR.
248151	pLVX-EF1_-EGFR-FCRL3-140aa-Chimera-puro	Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its full-length cytoplasmic tail, fused to the extracellular domain of EGFR.
248152	pLVX-EF1_-FCRL3-16aa-puro	Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 16 residues of the cytoplasmic tail.
248153	pLVX-EF1_-FCRL3-50aa-puro	Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 50 residues of the cytoplasmic tail.
248154	pLVX-EF1_-FCRL3-93aa-puro	Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 93 residues of the cytoplasmic tail.
248155	pLVX-EF1_-FCRL3-3xFLAG-puro	Lentiviral vector expressing human FCRL3 with 3xFLAG tags