SHARK: A Specialized Host for Assembling R6K Plasmids.
Hina-Nilesh Joshi S, Jenkins C, Ulaeto D, Gorochowski TE
ACS Synth Biol. 2026 Jan 13. doi: 10.1021/acssynbio.5c00749.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248166 | pR6K-mScarI | R6K plasmid with constituitive red fluorescent protein (mScarlet-I) expression under J23101 promoter |
| 248168 | pSHARK2 | pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-B0033-PirWT) expression |
| 248169 | pSHARK6 | pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-RJ64-Pir4150) expression |
| 248172 | pREMORAv1 | pSC101-ts plasmid with salicylic acid inducible Lambda-RED operon and constituitive recA expression |
| 248173 | SHARK2 | Maintenance of R6K plasmids at 13 PCN |
| 248174 | SHARK6 | Maintenance of R6K plasmids at 59 PCN |
| 248175 | SHARK7 | Maintenance of R6K plasmids at 95 PCN |
| 248176 | SHARK8 | Maintenance of R6K plasmids at 15 PCN |