Influence of expression and purification protocols on Galpha biochemical activity: kinetics of plant and mammalian G protein cycles.
Gookin TE, Chakravorty D, Assmann SM
Front Mol Biosci. 2025 Apr 7;12:1513660. doi: 10.3389/fmolb.2025.1513660. eCollection 2025.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248647 | pSTTa | E. coli protein expression vector encoding 2x N-terminal StrepII tags flanking an 11 residue flexible linker, thrombin and TEV protease sites, and an optional C-terminal FLAG tag. |
| 248648 | pSTTa-GPA1 | Vector for protein expression of 2x StrepII-tagged Arabidopsis heterotrimeric G protein GPA1 in E. coli. |
| 248649 | pSTTa-GPA1-S52N | Vector for protein expression of 2x StrepII-tagged Arabidopsis heterotrimeric G protein GPA1-S52N ("inactive" mutant) in E. coli. |
| 248650 | pSTTa-GPA1-Q222L | Vector for protein expression of 2x StrepII-tagged Arabidopsis heterotrimeric G protein GPA1-Q222L ("constitutively active" mutant) in E. coli. |
| 248651 | pSTTa-GNAI1 | Vector for protein expression of 2x StrepII-tagged human heterotrimeric G protein GNAI1 in E. coli. |
| 248652 | pSTTa-GNAO1 | Vector for protein expression of 2x StrepII-tagged human heterotrimeric G protein GNAO1 in E. coli. |