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Discovery and protein language model-guided design of hyperactive transposases.

Ivancic D, Agudelo A, Lindstrom-Vautrin J, Jaraba-Wallace J, Gallo M, Das R, Ragel A, Herrero-Vicente J, Higueras I, Billeci F, Sanvicente-Garcia M, Petazzi P, Ferruz N, Sanchez-Mejias A, Guell M
Nat Biotechnol. 2025 Oct 2. doi: 10.1038/s41587-025-02816-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248198pCMV_PB_PoeturPoetur transposase expressing plasmid
248199pCMV_PB_Antgra4Antgra4 transposase expressing plasmid
248200excision_reporter_HyPBHyPB and synthetic transposase excision reporter and targeted integration reporter
248201pTIR_HyPB_RFPHyPB RFP cargo plasmid
248202pTIR_Poetur_RFPPoetur RFP cargo plasmid
248203pTIR_Antgra4_RFPAntgra4 RFP cargo plasmid

Antibodies from Article