tRNA-Based Polycistronic CRISPR/Cas9 System Boosts Efficiency of Multi-Gene Deletion in the Moss Physcomitrella
Kozgunova E
bioRxiv 2025.07.29.667574
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248333 | EKv376(gRNAscaffold-tRNA for cloning) | Plasmid contains gRNA scaffold-tRNA and is used to amplify fragments for Golden Gate assembly |
| 248334 | EKv405(Gold Cas-guide insert1) | gRNA insert vector for Golden Gate Assembly |
| 248335 | EKv406(Gold Cas-guide insert2) | gRNA insert vector for Golden Gate Assembly |
| 248336 | EKv407(Gold Cas-guide insert3) | gRNA insert vector for Golden Gate Assembly |
| 248337 | EKv408(Gold Cas-guide insert4) | gRNA insert vector for Golden Gate Assembly |
| 248338 | EKv409(Gold Cas-guide insert5) | gRNA insert vector for Golden Gate Assembly |
| 248339 | EKv410(Gold Cas-guide insert6) | gRNA insert vector for Golden Gate Assembly |
| 248340 | EKv411(Gold Cas-guide insert7) | gRNA insert vector for Golden Gate Assembly |
| 248341 | EKv412(Gold Cas-guide insert8) | gRNA insert vector for Golden Gate Assembly |
| 248342 | EKv413(Gold Cas-guide insert4 2 gene version) | gRNA insert vector for Golden Gate Assembly, 2 gene or 4 gRNAs version |
| 248343 | EKv414(Gold Cas-guide insert6 3 gene version) | gRNA insert vector for Golden Gate Assembly, 3 gene or 6 gRNAs version |
| 248344 | EKv444(pIhcsrI677-Cas9 GG destination vector ) | Golden Gate destination vector containing S. pyogenes Cas9 nuclease (with BsaI sites removed). For multicassette gRNA assembly and expression in moss Physcomitrium patens (Physcomitrella). |