A programmable genetic platform for engineering noninvasive biosensors.
Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K, Tiwari R, Mukherjee A
Sci Adv. 2026 Jan 16;12(3):eaec1211. doi: 10.1126/sciadv.aec1211. Epub 2026 Jan 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248354 | pANC61(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron-IRES-EGFP) | Lentiviral vector that can express hAqp1_degron based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP marker |
| 248355 | pANC56(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-KKYL-IRES-EGFP) | Lentiviral vector that can express hAqp1_ER export based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP marker |
| 248356 | pANC79a(P4-GGGSGGG-cTEV-HA-IRES-dTomato) | Lentiviral vector that can express P4 along with C terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker |
| 248357 | pANC79b(P3-Myc-nTEV-IRES-EBFP) | Lentiviral vector that can express P3 along with N terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker |
| 248358 | pANC98a(3xFLAG-NES-FKBP12-(G4S)4-nTEV-IRES-dTomato) | Lentiviral vector that can express FKBP12 along with N terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker |
| 248359 | pANC98c(3xFLAG-NES-FRB-(G4S)4-cTEV(219)-IRES-EBFP) | Lentiviral vector that can express FRB along with a truncated C terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker |
| 248360 | pNTA-uTEV3-GSG-t2A-EBFP | Lentiviral vector that can express uTEV3. Expressed off of CMV tet ON promoter with EBFP marker |
| 248361 | pANC75(HCV NS3p-IRES-dTomato) | Lentiviral vector that can express HCV NS3p. Expressed off of CMV tet ON promoter with dTomato marker |
| 248362 | pANC76(TVMVp-IRES-dTomato) | Lentiviral vector that can express TVMVp. Expressed off of CMV tet ON promoter with dTomato marker |
| 248363 | pJW53(uTEV3-DHFR2.0-IRES-mCherry) | Lentiviral vector that can express destabilized uTEV3. Expressed off of CMV promoter with mCherry marker |