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The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use in E. coli.

Mejlsted J, Kubaczka E, Wirth S, Koeppl H
ACS Synth Biol. 2026 Jan 25. doi: 10.1021/acssynbio.5c00489. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248364pCTK001Encodes PAmeR as a Type 2ab part to be used in the CTK/YTK systems
248365pCTK002Encodes PAmtR as a Type 2ab part to be used in the CTK/YTK systems
248366pCTK003Encodes PBAD as a Type 2ab part to be used in the CTK/YTK systems
248367pCTK004Encodes PBetI as a Type 2ab part to be used in the CTK/YTK systems
248368pCTK005Encodes PBM3R1 as a Type 2ab part to be used in the CTK/YTK systems
248369pCTK006Encodes PHlyIIR as a Type 2ab part to be used in the CTK/YTK systems
248370pCTK007Encodes PIcaRA as a Type 2ab part to be used in the CTK/YTK systems
248371pCTK008Encodes PLitR as a Type 2ab part to be used in the CTK/YTK systems
248372pCTK009Encodes PLmrA as a Type 2ab part to be used in the CTK/YTK systems
248373pCTK010Encodes PPhlF as a Type 2ab part to be used in the CTK/YTK systems
248374pCTK011Encodes PPsrA as a Type 2ab part to be used in the CTK/YTK systems
248375pCTK012Encodes PQacR as a Type 2ab part to be used in the CTK/YTK systems
248376pCTK013Encodes PSrpR as a Type 2ab part to be used in the CTK/YTK systems
248377pCTK014Encodes PTac as a Type 2ab part to be used in the CTK/YTK systems
248378pCTK015Encodes PTet as a Type 2ab part to be used in the CTK/YTK systems
248379pCTK016Encodes PLacI as a Type 2ab part to be used in the CTK/YTK systems
248380pCTK017Encodes PAmtR as a Type 2a part to be used in the CTK/YTK systems
248381pCTK018Encodes PBAD as a Type 2a part to be used in the CTK/YTK systems
248383pCTK020Encodes PPhlF as a Type 2a part to be used in the CTK/YTK systems
248385pCTK022Encodes PTac as a Type 2a part to be used in the CTK/YTK systems
248387pCTK024Encodes PAmeR as a Type 2b part to be used in the CTK/YTK systems
248388pCTK025Encodes PAmtR as a Type 2b part to be used in the CTK/YTK systems
248390pCTK027Encodes PhlyIIR as a Type 2b part to be used in the CTK/YTK systems
248392pCTK029Encodes J23101 as a Type 2ab part to be used in the CTK/YTK systems
248393pCTK030Encodes J23102 as a Type 2ab part to be used in the CTK/YTK systems
248394pCTK031Encodes J23103 as a Type 2ab part to be used in the CTK/YTK systems
248395pCTK032Encodes J23104 as a Type 2ab part to be used in the CTK/YTK systems
248396pCTK033Encodes J23105 as a Type 2ab part to be used in the CTK/YTK systems
248397pCTK034Encodes J23106 as a Type 2ab part to be used in the CTK/YTK systems
248398pCTK035Encodes J23107 as a Type 2ab part to be used in the CTK/YTK systems
248399pCTK036Encodes J23108 as a Type 2ab part to be used in the CTK/YTK systems
248400pCTK037Encodes J23109 as a Type 2ab part to be used in the CTK/YTK systems
248401pCTK038Encodes J23110 as a Type 2ab part to be used in the CTK/YTK systems
248403pCTK040Encodes J23113 as a Type 2ab part to be used in the CTK/YTK systems
248404pCTK041Encodes J23115 as a Type 2ab part to be used in the CTK/YTK systems
248406pCTK043Encodes J23117 as a Type 2ab part to be used in the CTK/YTK systems
248407pCTK044Encodes J23118 as a Type 2ab part to be used in the CTK/YTK systems
248408pCTK045Encodes J23119 as a Type 2ab part to be used in the CTK/YTK systems
248409pCTK046Encodes BydvJ as a Type 2c part to be used in the CTK/YTK systems
248410pCTK047Encodes ElvJ as a Type 2c part to be used in the CTK/YTK systems
248411pCTK048Encodes PlmJ as a Type 2c part to be used in the CTK/YTK systems
248413pCTK050Encodes RiboJ10 as a Type 2c part to be used in the CTK/YTK systems
248414pCTK051Encodes RiboJ51 as a Type 2c part to be used in the CTK/YTK systems
248415pCTK052Encodes RiboJ53 as a Type 2c part to be used in the CTK/YTK systems
248416pCTK053Encodes RiboJ54 as a Type 2c part to be used in the CTK/YTK systems
248417pCTK054Encodes RiboJ57 as a Type 2c part to be used in the CTK/YTK systems
248418pCTK055Encodes RiboJ60 as a Type 2c part to be used in the CTK/YTK systems
248419pCTK056Encodes SarJ as a Type 2c part to be used in the CTK/YTK systems
248420pCTK057Encodes ScmJ as a Type 2c part to be used in the CTK/YTK systems
248421pCTK058Encodes B0064 as a Type 2d part to be used in the CTK/YTK systems
248422pCTK059Encodes RBS-A1 as a Type 2d part to be used in the CTK/YTK systems
248423pCTK060Encodes RBS-B1 as a Type 2d part to be used in the CTK/YTK systems
248424pCTK061Encodes RBS-B2 as a Type 2d part to be used in the CTK/YTK systems
248425pCTK062Encodes RBS-B3 as a Type 2d part to be used in the CTK/YTK systems
248426pCTK063Encodes RBS-E1 as a Type 2d part to be used in the CTK/YTK systems
248427pCTK064Encodes RBS-F1 as a Type 2d part to be used in the CTK/YTK systems
248428pCTK065Encodes RBS-H1 as a Type 2d part to be used in the CTK/YTK systems
248429pCTK066Encodes RBS-I1 as a Type 2d part to be used in the CTK/YTK systems
248430pCTK067Encodes RBS-L1 as a Type 2d part to be used in the CTK/YTK systems
248431pCTK068Encodes RBS-N1 as a Type 2d part to be used in the CTK/YTK systems
248432pCTK069Encodes RBS-P1 as a Type 2d part to be used in the CTK/YTK systems
248433pCTK070Encodes RBS-P2 as a Type 2d part to be used in the CTK/YTK systems
248434pCTK071Encodes RBS-P3 as a Type 2d part to be used in the CTK/YTK systems
248435pCTK072Encodes RBS-Q1 as a Type 2d part to be used in the CTK/YTK systems
248436pCTK073Encodes RBS-Q2 as a Type 2d part to be used in the CTK/YTK systems
248437pCTK074Encodes RBS-R1 as a Type 2d part to be used in the CTK/YTK systems
248438pCTK075Encodes RBS-S1 as a Type 2d part to be used in the CTK/YTK systems
248439pCTK076Encodes RBS-S2 as a Type 2d part to be used in the CTK/YTK systems
248440pCTK077Encodes RBS-S3 as a Type 2d part to be used in the CTK/YTK systems
248441pCTK078Encodes RBS-S4 as a Type 2d part to be used in the CTK/YTK systems
248442pCTK079Encodes mCherry as a Type 3 part to be used in the CTK/YTK systems
248443pCTK080Encodes sfGFP as a Type 3 part to be used in the CTK/YTK systems
248445pCTK082Encodes ameR as a Type 3 part to be used in the CTK/YTK systems
248446pCTK083Encodes amtR as a Type 3 part to be used in the CTK/YTK systems
248447pCTK084Encodes betI as a Type 3 part to be used in the CTK/YTK systems
248448pCTK085Encodes bm3R1 as a Type 3 part to be used in the CTK/YTK systems
248449pCTK086Encodes hlyIIR as a Type 3 part to be used in the CTK/YTK systems
248450pCTK087Encodes icaRA as a Type 3 part to be used in the CTK/YTK systems
248451pCTK088Encodes litR as a Type 3 part to be used in the CTK/YTK systems
248452pCTK089Encodes lmrR as a Type 3 part to be used in the CTK/YTK systems
248453pCTK090Encodes phlF as a Type 3 part to be used in the CTK/YTK systems
248454pCTK091Encodes psrA as a Type 3 part to be used in the CTK/YTK systems
248455pCTK092Encodes qacR as a Type 3 part to be used in the CTK/YTK systems
248456pCTK093Encodes srpR as a Type 3 part to be used in the CTK/YTK systems
248457pCTK094Encodes lacI as a Type 3 part to be used in the CTK/YTK systems
248458pCTK095Encodes TetR as a Type 3 part to be used in the CTK/YTK systems
248459pCTK096Encodes mCherry as a Type 3a part to be used in the CTK/YTK systems
248460pCTK097Encodes sfGFP as a Type 3a part to be used in the CTK/YTK systems
248495pCTK132Encodes PTet-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Antibodies from Article