Optogenetic control of mechanotransduction based on light-induced homodimerization of talin.
Nishimura R, Barnett SFH, Jain K, Huang Z, Goult BT, Kanchanawong P
J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249166 | pEGFP-N1/Integrin-b5-FL-SGx3-Halo9 | Mammalian expression vector of human ITGB5 fused with Halo9-tag |
| 249167 | mApple-Talin1(1–1973)-pdDronpa1.2 | Mammalian expression vector of talin1 N-terminal fragment fused with pdDronpa1.2 |
| 249168 | pdDronpa1.2-Talin1(1974–2541)-mIFP | Mammalian expression vector of talin1 C-terminal fragment fused with pdDronpa1.2 |
| 249169 | mIFP-Talin1(1–2493) | Mammalian expression vector of truncated talin1 lacks C-terminal dimerization domain (ΔDD) |
| 249170 | mIFP-Talin1(1–2493)-pdDronpa1.2 | Mammalian expression vector of opto-dimerizable talin1 |
| 249171 | pEGFP-N1/YAP-mScarlet3-H | Mammalian expression vector of YAP fused with mScarlet3-H |
| 249173 | pEGFP-N1/Halo-CAAX | Mammalian expression vector of CAAX motif (plasma membrane marker) fused with Halo-tag |