Alice Ting Lab: Ting Lab LplA Plasmids 2025

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
248801	His6-mCherry-Flag-LplA Wild Type	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248803	His6-mCherry-Flag-LplAW37V and L172A	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248804	His6-mCherry-Flag-LplAW37V and P167I	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248806	His6-mCherry-Flag-LplAW37V and T57I	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248808	His6-mCherry-Flag-LplAW37V and F147S	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248809	His6-mCherry-Flag-LplAW37V and A48R	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248810	His6-mCherry-Flag-LplAW37V and Y139S	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248811	His6-mCherry-Flag-LplAW37V and A48N	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248812	His6-mCherry-Flag-LplA W37V and E265R	Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
248813	His6-TEV-LplA	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248814	His6-TEV-LplA W37V	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248815	His6-TEV-LplA W37V and L172A	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248816	His6-TEV-LplA W37V and P167I	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248818	His6-TEV-LplA W37V and T57I	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248819	His6-TEV-LplA W37V and C55M	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248820	His6-TEV-LplA W37V and F147S	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248821	His6-TEV-LplA W37V and A48R	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248822	His6-TEV-LplA W37V and Y139S	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248823	His6-TEV-LplA W37V and A48N	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248824	His6-TEV-LplA W37V and E265R	Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
248825	His-LAP-BFP-CAAX	Plasmid used to express LplA's site-specific substrate LAP2 (13-amino acid acceptor peptide) in mammalian cells. CAAX targets it to the inner leaflet of the plasma membrane.
248826	His-LAP-BFP-NLS	LAP2 fused to BFP and NLS, a nuclear localization signal. Imaging shows primarily nucleolar localization.
248827	His-HA-E2p-YFP	Plasmid used to express E2p, a site-specific acceptor protein for LplA, in HEK293T cells. Used to evaluate specific labeling activity of LplA variants.