Investigating morphogen and patterning dynamics with optogenetic control of morphogen production.
Benzinger D, Briscoe J
Dev Cell. 2025 Aug 19:S1534-5807(25)00495-2. doi: 10.1016/j.devcel.2025.07.019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 248962 | pDBB31 | DD-GAVPO (degron-regulated GAVPO) expression under control of the EF1alpha promoter in a PiggyBac integration plasmid |
| 248963 | pDBB56 | Optogenetic expression of Shh driven by five GAL4/GAVPO binding sites in front of a synthetic YB-TATA minimal promoter in a PiggyBac integration plasmid. |
| 248966 | pDBB63 | Optogenetic expression of NShh-CD4 driven by five GAL4/GAVPO binding sites in front of a synthetic YB-TATA minimal promoter in a PiggyBac integration plasmid. |
| 248967 | PBgg-Puro | Golden Gate assembly-friendly PiggyBac backbone with Puromycin resistance |
| 248968 | PBgg-Hygro | Golden Gate assembly-friendly PiggyBac backbone with Hygromycin resistance |