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Adapting CRISPR-associated transposons for rapid and high-throughput reverse genetics.

Basta DW, Zingl FG, Yang Y, Nguyen KT, Liu YY, Waldor MK
bioRxiv [Preprint]. 2025 Nov 1:2025.10.31.685825. doi: 10.1101/2025.10.31.685825. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
248973MultiCAST-HelperMultiCAST helper plasmid encoding CAST Tn6677 CRISPR and transposition proteins
248974MultiCAST-miniTn-KanMultiCAST mini-Tn donor plasmid containing kanamycin selectable marker
248975MultiCAST-miniTn-CarbMultiCAST mini-Tn donor plasmid containing ampicillin/carbenicillin selectable marker

Antibodies from Article