HSP90 buffers deleterious genetic variations in BRCA1.

Gracia B, Zhang XH, Montes P, Pham TC, Huang M, Chen J, Karras GI
Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 10.1016/j.molcel.2025.10.026. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
248988	pKlab897-HBEGF-2a-3xFLAG-BRCA1(full-length)-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 protein sequence, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248989	pKlab1069-HBEGF-2a-3xFLAG-BRCA1(full-length)-A1708V-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with A1708V mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248990	pKlab1066-HBEGF-2a-3xFLAG-BRCA1(full-length)-L1705R-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with L1705R mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248991	pKlab1070-HBEGF-2a-3xFLAG-BRCA1(full-length)-Y1845D-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with Y1845D mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248992	pKlab1067-HBEGF-2a-3xFLAG-BRCA1(full-length)-S1715R-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with S1715R mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248993	pKlab1153-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1692N-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with D1692N mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248994	pKlab1154-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1692H-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with D1692H mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248995	pKlab1155-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1739V-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with D1739V mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248996	pKlab1156-HBEGF-2a-3xFLAG-BRCA1(full-length)-M1783T-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with M1783T mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248997	pKlab1157-HBEGF-2a-3xFLAG-BRCA1(full-length)-P1749S-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with P1749S mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248998	pKlab1158-HBEGF-2a-3xFLAG-BRCA1(full-length)-R1699Q-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with R1699Q mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
248999	pKlab1159-HBEGF-2a-3xFLAG-BRCA1(full-length)-R1699W-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with R1699W mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
249000	pKlab1160-HBEGF-2a-3xFLAG-BRCA1(full-length)-S1655F-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with S1655F mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
249001	pKlab1161-HBEGF-2a-3xFLAG-BRCA1(full-length)-V1736A-2a-mRuby3-HBBIVS2-WPRE	This plasmid expresses the BRCA1 sequence with V1736A mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
249002	pKlab1194-pcDNA-3.1-BACH1/BRIP1/FANCJ-3xFLAG-V5	This plasmid expresses the BACH1 protein sequence for pull down assays
249003	pKlab1197-pcDNA-3.1-3xFLAG-V5-hPALB2 (T333A, L703L, H1184R)	This plasmid expresses the hPALB2 protein seqence for pull down assays
249004	pKlab966-pUC19-HAL-BRCA1-BSD-P2A-2xHA-FKBP12-F36V-HAR-BRCA1	This plasmid expresses the degron tag knock-in sequence at the N-terminus of the endogenous BRCA1 locus
249005	pKlab965-PX459-BRCA1-Nterm-guide1	This plasmid expresses the Cas9/gRNA that will target the HBEGF gene locus
249007	pKlab821-pcDNA3.1-HALO-3xFLAG	This plasmid expresses the C terminally 3x-FLAG tagged HALO protein sequences for pulldown assays
249023	pKlab810-bGlobin_3xFLAG-BRCA1(full-length)	This plasmid expresses the full length BRCA1 sequence with an N-terminal 3xFLAG tag
249024	pKlab813-bGlobin_3xF-BRCA1(full-length)-Y1845D	This plasmid expresses the full length BRCA1 sequence (Y1845D mutation) with an N-terminal 3xFLAG tag
249025	pKlab814-bGlobin_3xF-BRCA1(full-length)-M1775R	This plasmid expresses the full length BRCA1 sequence (M1775R mutation) with an N-terminal 3xFLAG tag
249026	pKlab816-bGlobin_3xF-BRCA1(full-length)-G1788V	This plasmid expresses the full length BRCA1 sequence (G1788V mutation) with an N-terminal 3xFLAG tag
249027	pKlab818-bGlobin_3xF-BRCA1(full-length)-A1708E	This plasmid expresses the full length BRCA1 sequence (A1708E mutation) with an N-terminal 3xFLAG tag
249028	pKlab819-bGlobin_3xF-BRCA1(full-length)-Y1845F	This plasmid expresses the full length BRCA1 sequence (Y1845F mutation) with an N-terminal 3xFLAG tag
249029	pKlab676-HBEGF-2a-mWasabi-WPRE	This plasmid expresses the mWasabi flourescent tag for integration into mammalian cell lines
249030	pKlab639-HBEGF-2a-mRuby3-WPRE	This plasmid expresses the mRuby3 flourescent tag for integration into mammalian cell lines
249031	pKlab672-HBEGF-2a-mTagBFP2-WPRE	This plasmid expresses the mTagBFP2 flourescent tag for integration into mammalian cell lines