Local gene editing of fibroblasts in tumors reveals a new cancer-associated fibroblast state.
Kuhn NF, Zaleta-Linares I, Hu KH, Courau T, Davidson B, Tam T, Chu CW, Kinet MJ, Rosenblum MD, Combes AJ, Nyberg WA, Eyquem J, Krummel MF
J Exp Med. 2026 May 4;223(5):e20251228. doi: 10.1084/jem.20251228. Epub 2026 Mar 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249119 | pscAAV-U6-gCtnnb1-CBh-mCherry-SV40late | expression of gCtnnb1 and mCherry |
| 249120 | pscAAV-U6-gCxl5-CBh-mCherry-SV40late | expression of gCxcl5 and mCherry |
| 249121 | pscAAV-U6-gIl1r1-CBh-mCherry-SV40late | expression of gIl1r1 and mCherry |
| 249122 | pscAAV-U6-gOsmr-CBh-mCherry-SV40late | expression of gOsmr and mCherry |
| 249123 | pscAAV-U6-gPdpn-CBh-mCherry-SV40late | expression of gPdpn and mCherry |
| 249124 | pscAAV-U6-gSCR-CBh-mCherry-SV40late | expression of gSCR (scrambled, non-targeting gRNA) and mCherry |
| 249125 | pscAAV-U6-gTgfbr2-CBh-mCherry-SV40late | expression of gTgfbr2 and mCherry |
| 249126 | pscAAV-U6-gThy1-CBh-mCherry-SV40late | expression of gThy1 and mCherry |
| 249127 | pscAAV-U6-gTnfrfs1a-CBh-mCherry-SV40late | expression of gTnfrsf1a and mCherry |
| 249128 | pscAAV-U6-gTrac-CBh-mCherry-SV40late | expression of gTrac and mCherry |
| 249129 | pscAAV-KIKO-U6-gThy1-p2a-mCherry-STOP-pA-400 | all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gThy1 cut site, disrupting Thy1 expression (=knockout) |
| 249130 | pscAAV-KIKO-U6-gOsmr-p2a-mCherry-STOP-pA-400 | all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gOsmr cut site, disrupting Osmr expression |
| 249131 | pscAAV-KIKO-U6-gTgfbr2-p2a-mCherry-STOPpA-400 | all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gTgfbr2 cut site, disrupting Osmr expression (=knockout) |