Heterochromatome wide analyses reveal MBD2 as a phase separation scaffold for heterochromatin compartmentalization and composition.

Zhang H, Ugur E, Hake C, Romero H, Arroyo M, Mahmoud M, Lermyte F, Leonhardt H, Cardoso MC
Nucleic Acids Res. 2025 Nov 26;53(22):gkaf1380. doi: 10.1093/nar/gkaf1380. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
229559	pMBD2-1-GFP pc2067	Mammalian expression vector codes for MBD2(aa 1-152) fused c-terminal to GFP.
229560	pmMBD2.2-GFP pc2068	Mammalian expression vector codes for MBD2(aa 153-414) fused c-terminal to GFP.
229561	pmMBD2.4G pc2841	Mammalian expression vector codes for MBD2(aa 235-414) fused c-terminal to GFP.
229562	pmMBD2-6G pc2843	Mammalian expression vector codes for MBD2(aa 153-235) fused c-terminal to GFP.
229748	pTYB1-MBD1∆CxxC3 pc4783	Bacterial expression vector of MBD1 with a deletion of CXXC3 domain. C-terminal tagged to the intein.
229749	pTYB1-MBD3 pc4784	Bacterial expression vector of MBD3 C-terminal tagged to the intein.
229750	pTYB1-MBD4 pc4785	Bacterial expression vector of MBD4 C-terminal tagged to the intein.
229751	pTYB1-MBD2 pc4786	Bacterial expression vector of MBD2 C-terminal tagged to the intein.
229752	pTYB1-MBD2∆N(MBD2b) pc4787	Bacterial expression plasmid of MBD2∆N(MBD2b) lacking the N-terminus: aa 1-152. C-terminal tagged to intein.
229753	pTYB1-MBD2-N pc4789	Bacterial expression plasmid of MBD2-N (N-terminus of MBD2: aa 1-152). C-terminal tagged to intein.
229754	pTYB1-MBD2-MBDTRD pc4791	Bacterial expression vector of MBD2-MBDTRD domains (aa 153-235) C-terminal tagged to the intein.
229755	pTYB1-MBD2∆C pc4792	Bacterial expression plasmid of MBD2∆C lacking the C-terminus (a 236-414). C-terminal tagged to intein.
229756	pTYB1-MBD2-C pc4793	Bacterial expression plasmid of MBD2-C (C-terminus of MBD2: aa 236-414). C-terminal tagged to intein.
229757	pmMBD2∆C-G pc4794	Mammalian expression vector of MBD2. The Protein is lacking the C-terminus: aa 236-414. C-terminal tagged to GFP.
229758	pTYB1-MBD2∆GR pc5078	Bacterial expression plasmid of MBD2∆GR. The Protein is lacking the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.
229759	pTYB1-MBD2∆C∆GR pc5079	Bacterial expression plasmid of MBD2∆C∆GR. MBD2 lacking the C-terminus of MBD2: aa 236-414 and the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.
229760	pTYB1-MBD2a∆CC pc5081	Bacterial expression vector of MBD2a lacking the coiled coil domain: aa 363-396.
229761	pTYB1-MBD2-C∆CC pc5084	Bacterial expression plasmid of MBD2-C∆CC. C-terminus of MBD2 is lacking the coiled coil domain: aa 363-396. C-terminal tagged to intein.
229762	pmMBD2∆C∆GR-G pc5086	Mammalian expression plasmid of MBD2-∆C∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to GFP
229763	pmMBD2-N∆GR-G pc5087	Mammalian expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to GFP.
229764	pmMBD2∆CC-G pc5088	Mammalian expression plasmid of MBD2∆CC. Protein is lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.
229765	pmMBD2∆N∆CC-G pc5089	Mammalian expression plasmid of MBD2∆N∆CC. Protein is lacking the coiled coil domain: aa 363-396 and the N-terminus: aa 1-152. C-terminal tagged to GFP.
229766	pmMBD2-C∆CC-G pc5091	Mammalian expression plasmid of MBD2-C∆CC. C-terminus of MBD2 lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.
230974	pTYB1-MBD2-N∆GR (pc5083)	Bacterial expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to intein.