Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes.

Gupta D, Patinios C, Bassett HV, Kibe A, Collins SP, Kamm C, Wang Y, Zhao C, Vollen K, Toussaint C, Calvin I, Cullot G, Aird EJ, Polkoff KM, Nguyen-Vo TP, Migur A, Schut F, Al'Abri IS, Achmedov T, Del Re A, Corn JE, Saliba AE, Crook N, Stepanova AN, Alonso JM, Beisel CL
Nat Biotechnol. 2025 Sep 4. doi: 10.1038/s41587-025-02802-w. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
249324	pCBS-5738	To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing
249325	pCBS-5753	To express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing
249326	pCBS-5737	Expresses Non-targeting sgRNA