Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes.
Gupta D, Patinios C, Bassett HV, Kibe A, Collins SP, Kamm C, Wang Y, Zhao C, Vollen K, Toussaint C, Calvin I, Cullot G, Aird EJ, Polkoff KM, Nguyen-Vo TP, Migur A, Schut F, Al'Abri IS, Achmedov T, Del Re A, Corn JE, Saliba AE, Crook N, Stepanova AN, Alonso JM, Beisel CL
Nat Biotechnol. 2025 Sep 4. doi: 10.1038/s41587-025-02802-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249320 | pCBS-4804 | Expresses Non-targeting sgRNA and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655 |
| 249321 | pCBS-4803 | Expresses an sgRNA to target the KanR* site on the engineered E. coli MG1655 strain and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655 |
| 249322 | pCBS-6738 | To express the EPEC DarT2(G49D)-nScCas9(D10A) fusion protein in experiments for Append Editing |
| 249323 | pCBS-4800 | To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing |
| 249324 | pCBS-5738 | To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing |
| 249325 | pCBS-5753 | To express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing |
| 249326 | pCBS-5737 | Expresses Non-targeting sgRNA |
| 249327 | pCBS-5740 | Expresses sgRNA targeting the fcy gene in S. cerevisiae |
| 249328 | pCBS-6756 | Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cells |
| 249329 | pCBS-5846 | Expresses EPEC DarT2(G49D)-nSpCas9(D10A)and sg#3 targeting VEGFA gene in mammalian cells |
| 249330 | pCBS-5391 | Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and Non-targeting guide for mammalian cells |
| 249331 | pCBS-4559 | Expresses EPEC DarT2(G49D)-nSpCas9(D10A) in mammalian cells and gfp-dropout cassette for cloning sgRNAs |