Reconstitution of multistep recruitment of ULK1 to membranes in autophagy.
Duan Y, Lu Y, Paul S, Betz J, Wilhelm L, Cook ASI, Ren X, Adriaenssens E, Martens S, Ganley IG, Hummer G, Hurley JH
bioRxiv [Preprint]. 2025 Nov 9:2025.11.07.687251. doi: 10.1101/2025.11.07.687251.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249533 | pCAG-TSF-ULK1 | Expressing human full length ULK1 |
| 249534 | pCAG-TSF-ULK1-ADA | Expressing human full length ULK1 with P433A/V434D/P435A mutations |
| 249535 | pBMN-HA-ATG13-HF|DD | Expressing human full length ATG13 with H214D/F215D mutations |
| 249729 | pCAG-MBP-ULK1-1-835-mcherry | Expressing human ULK1-1-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal |
| 249732 | pCAG-MBP-ULK1-1-290-mcherrry | Expressing human ULK1-1-290 with a MBP tag in N-terminal and a mcherry tag in C-terminal |
| 249733 | pCAG-MBP-ULK1-290-835-mcherry | Expressing human ULK1-290-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal |
| 250340 | pCAG-ATG101ΔCTH | Expression human ATG101ΔCTH |
| 250341 | pCAG-TSF-ATG101ΔCTH (WF|DD) | Expressing human ATG101ΔCTH with W110D and F112D mutation |
| 250342 | pCAG-GST-ATG101(WF|DD) | Expressing human full length ATG101 with W110D and F112D mutations |
| 250343 | pCAG-GFP-ATG13-1-230 | Expressing human ATG13 (aa. 1-230) |
| 250344 | pCAG-GFP-ATG13-1-230(HF|DD) | Expressing human ATG13 (1-230) with H214D/F215D mutations |