An autism spectrum disorder mutation in Topoisomerase 3beta causes accumulation of covalent mRNA intermediates by disrupting metal binding within the zinc finger domain.
Warrick JE, Attili D, van Eeuwen T, Pastore B, Hoffmann-Weitsman SE, Forsyth NC, Tang W, Barmada SJ, Kearse MG
Nucleic Acids Res. 2025 Oct 28;53(20):gkaf1138. doi: 10.1093/nar/gkaf1138.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249681 | pCMV6-TOP3B (WT)-3xFLAG | Expresses human TOP3B (wildtype) with a C-terminal 3xFLAG tagged in mammalian cells |
| 249682 | pCMV6-TOP3B (Y336F)-3xFLAG | Expresses human TOP3B (Y336F; catalytically inactive mutant) with a C-terminal 3xFLAG tag in mammalian cells |
| 249683 | pCMV6-TOP3B (R338W)-3xFLAG | Expresses human TOP3B (R338W; "self-trapping" mutant) with a C-terminal 3xFLAG tag in mammalian cells |