Expanding the genetic toolkit: adenine and cytosine base editors for gene disruption in Aspergillus niger
Yuan G, Deng S, Dai Z, Hofstad BA, Pomraning KR
Microb Cell Fact (2026). doi: 10.1186/s12934-026-02979-y
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249718 | pGY40 | pGY40 is a CRISPR cytosine base editing vector with nCas9 and sgRNA cassette, enabling targeted C-to-T conversions at NGG PAM sites in filamentous fungi. |
| 249719 | pGY49 | pGY49 is a CRISPR adenine base editing vector with nCas9 and sgRNA cassette, enabling targeted A-to-G conversions at NGG PAM sites in filamentous fungi. |
| 249722 | pGY150 | pGY150 is a CRISPR adenine base editing vector with the nickase variant of SpCas9-NG (nCas9-NG) and an sgRNA cassette, enabling targeted A-to-G conversions at flexible NG PAM sites in filamentous fung |
| 249723 | pGY151 | pGY151 is a CRISPR cytosine base editing (CBE) vector with the nickase variant of SpCas9-NG (nCas9-NG) and an sgRNA cassette, enabling targeted C-to-T conversions at flexible NG PAM sites in filamento |