A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants
Lavilla-Puerta M, He Y, Piccinini L, Di Paco L, Papachristodoulou A, Licausi F, Giuntoli B
bioRxiv 2025.09.01.673502
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249734 | HRPEadh-pAG305 | Derived from pAG305GPD-ccdb (#14138); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs. |
| 249735 | HRPEadh-pAG303 | Derived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs. |
| 249736 | pAG304-ccdbNLUC | Derived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiae |
| 249814 | SYRAP_pDONR201 | Encodes SYRAP (RAP2.12-GAL4STE12), Gateway compatible as Donor plasmid |
| 249815 | PCO4SV40_pDONR201 | Encodes PCO4 fused to a SV40-based NLS, Gateway compatible as Donor plasmid |
| 249816 | UbSYRAP_pDONR201 | Encodes UbSYRAP, a SYRAP split version (UBQ-RAP2.12-GAL4STE12), Gateway compatible as Donor plasmid |
| 249817 | UbSYHRE_pDONR201 | Encodes UbSYHRE (UBQ-HRE1-GAL4STE12), Gateway compatible as Donor plasmid |